Seminar: Atomic-Resolution Electron Microscopy for Seeing Molecular Motion, Assembly, and Reactions by Koji Harano

Seminar by Koji HARANO, Project Associate Professor
Department of Chemistry, The University of Tokyo

Tuesday, February 12th, 2019
2-3pm S1-02-02 (CBIS meeting room)

Abstract: Whereas a spatial- and time-average of molecular ensembles has been the conventional source of information on molecular structures, atomic resolution movies of single organic molecules and molecular clusters obtained by single-molecule atomic-resolution real-time electron microscopy (SMART-EM) developed by us have recently emerged as a new tool to study the time evolution of the structures of individual molecules. The key of our technique is that we observed little sign of decomposition of the specimen molecules encapsulated in or attached to a single-walled carbon nanotube (CNT) as opposed to solid organic materials.

Workshop on quantitative single molecule imaging with world experts

Workshop on quantitative single molecule imaging with world experts

March 26th to 28th
Mechanobiology Institute

Organizers
V. ViasnoffJ.B. SibaritaT. Wohland, G.Wright

Topics

  • Single molecule-based super-resolution microscopy
  • Single molecule localization and image reconstruction cluster analysis
  • Fluorescence correlation spectrocopy

Program

(Mornings: theory, Afternoons: hands-on (capped to 20 participants))

Tuesday March 26th

Morning

  • Fluorescence microscopy and single particle techniques (Eric Hosy, CNRS)
  • Fluorescence correlation spectroscopy (Thorsten Wohland, DBS)

Afternoon

  • Hands on parallel sessions: SM imaging/data analysis. Venue: MBI

Wednesday March 27th

Morning

  • Single molecule data quantification and image reconstruction (Jean-Bapiste Sibarita, CNRS)
  • Examples of super-resolution imaging capabilities within Singascope (Graham Wright, IMB)

Afternoon

  • Hands on parallel sessions: SM imaging/data analysis. Venue: MBI

Thursday March 28th

Morning

  • Advanced single molecule imaging (Jean-Bapiste Sibarita, CNRS)
  • TBA (Felix Margadant, NUS)
  • TBA (Tony Kanchanawong, NUS)
  • Probing transcription factors dynamics by FCS (Nicolas Plachta, IMCB)

Afternoon

  • Hands on FCS technics (single point, 2D). Venue: DBS

Registration is FREE but MANDATORY. Register here  or send an email to dbsvvnr@nus.edu.sg