CBIS Laboratory Facilities

The CBIS open labs are located on level 1 of building S1A and have been designed to encourage interdisciplinary interaction between approximately 80-90 faculty, staff, and students. Individuals from different labs are assigned at random to a lab bench and desk to encourage the maximum exchange of ideas.

CBIS Laboratory Equipment and Facilities

The Centre’s experimental floor is organized around five specially designed optical rooms housing custom designed and built microscopes, five core equipment rooms, a large tissue culture room, and a central space containing wet benches for 60 researchers. The close proximity of tissue culture, lab bench, and equipment rooms ensures rapid transfer of sample to microscopes.

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Lab tech Keshma Devan in the CBIS open lab at S1A.

The Centre’s communal wet benches are randomly assigned to researchers and students of different research groups, aimed to encourage interdisciplinary interactions and to foster scientific collaborations. The laboratory has five specially designed optical rooms, each housing a wide range of advanced microscopy systems, custom-built to specifications that meet the needs of each research group.

Besides the communal bench space, research equipment such as tissue culture hoods, deep freezers, centrifuges and incubators are also housed in the four equipment rooms and a large tissue culture room. These facilities are shared among all research groups. The close proximity between the wet benches, microscopy systems, tissue culture to other lab equipment enables the seamless and rapid transfer of samples.

CBIS currently houses the following imaging systems:

Prof Yan Jie’s group has two Olympus IX71s and one IX81 in CBIS for use in highly sensitive single-molecule manipulation and imaging techniques. The systems are modified to magnetic tweezers for single molecule study. Learn more about Yan Jie’s research.

Prof Thorsten Wohland’s lab has two confocal systems: the  Olympus FV1200 with a PicoQuant (TimeHarp 260) upgrade for single molecule detection (FCS, lifetime FLCS, FRET) and an Olympus FV300 with a home-built FCS/FCCS module.  His group also uses two TIRF systems: the Olympus cell^TIRF and an Olympus TIRF. Also in Prof Wohland’s lab is a home-built SPIM with imaging FCS functionality. Prof Wohland’s group investigates biomolecular actions and functions under physiological relevant conditions to gain insights into important biological questions. Read more about the Wohland lab’s research.

Abbreviations
FCS: Fluorescence Correlation Spectroscopy
FCCS: Fluorescence Cross-Correlation Spectroscopy
FLCS: Fluorescence Lifetime Correlation Spectroscopy
FRET: Förster Resonance Energy Transfer
SPIM: Single Plane Illumination Microscope
TIRF: Total Internal Reflection Microscopy

CBIS Cryoelectron Microscopy Facility

The Centre’s state-of-the-art Cryoelectron Microscopy Facility is located in the basement of building S2, a complete renovation of the former DBS Microscopy Facility. Find out more

CBIS Confocal Microscopy Laboratory

The Centre oversees the Department of Biological Sciences’ Confocal Microscopy Laboratory, which is located in S1A, level 3. Learn more