by Wu M, Wu X, De Camilli P
Proc Natl Acad Sci USA 2013 Jan 22;110(4):1339-44
Dynamic spatial patterns of signaling factors or macromolecular assemblies in the form of oscillations or traveling waves have emerged as important themes in cell physiology. Feedback mechanisms underlying these processes and their modulation by signaling events and reciprocal cross-talks remain poorly understood. In this work we show that antigen stimulation of mast cells triggers cyclic recruitment of curvature-generating protein FBP17 and actin regulatory factors that can be manifested in either spatial pattern. Further mechanistic studies revealed an unexpected pattern-rendering mechanism.
Read online: PNAS.
Learn more about Wu Min’s research.
by Lu Gan, Mark S Ladinsky, and Grant J Jensen
Chromosoma 2013 Jul 3
Chromatin organization is central to many conserved biological processes, but it is generally unknown how the underlying nucleosomes are arranged in situ. Here, we have used electron cryotomography to study chromatin in the picoplankton Ostreococcus tauri, the smallest known free-living eukaryote.
By visualizing the nucleosome densities directly, we find that O. tauri chromosomes do not arrange into discrete, compact bodies or any other higher level of order. In contrast to the textbook 30-nm-fiber model, O. tauri chromatin resembles a disordered assemblage of nucleosomes akin to the polymer-melt model. This disorganized nucleosome arrangement has important implications for potentially conserved functions in tiny eukaryotes such as the clustering of non-homologous chromosomes at the kinetochore during mitosis and the independent regulation of closely positioned adjacent genes.
Read online: Springer.
Learn more about Lu Gan’s research.
by Nirmalya Bag, Ashraf Ali, Virander Singh Chauhan, Thorsten Wohland and Aseem Mishra
Chem Commun 2013, 49, 9155–9157
Monomeric hIAPP significantly destabilizes both model and live cell membranes by increasing membrane fluidity. This interaction with membranes happens via carpet formation followed by lipid extraction in a concentration dependent manner and thus we propose that hIAPP aggregation prior to membrane interaction may not be necessary for its cytotoxicity.
‘FCS movie’ of live cell membranes upon exposure to the membrane-active peptide amylin
The lateral diffusion of the cell membrane is mapped by imaging fluorescence correlation spectroscopy (Imaging FCS) with 240 nm spatial resolution and 1 ms temporal resolution over a large membrane area (5´5 mm2). We created a 60-minute FCS time lapse movie to follow membrane dynamics upon interaction with amylin (hIAPP), a peptide involved in insulin secretion. The movie shows how the peptide interacts with the bilayer and changes diffusion patterns with time (blue areas forming with advancing time).
Read online: Royal Society Chemistry.
Learn more about Thorsten Wohland’s research.
by Joerg Renn, Anita Büttner, Thuy Thanh To, Sherlynn Jin Hui Chan and Christoph Winkler
Dev Biol 2013 Sep 1;381(1):134-43
In teleosts, such as medaka, ossification of the vertebral column starts with the mineralization of the notochordal sheath in a segmental pattern. This establishes the chordal centrum, which serves as the basis for further ossifications by sclerotome derived osteoblasts generating the vertebral body.
So far, it is unclear which cells produce the notochordal sheath and how a segmental pattern of mineralization is established in teleosts. Here, we use a transgenic medaka line that expresses nlGFP under the control of the col10a1 promoter for in vivo analysis of vertebral body formation. We show that col10a1:nlGFP expression recapitulates endogenous col10a1 expression.
In the axial skeleton, col10a1:nlGFP cells appear prior to the mineralization of the notochordal sheath in a segmental pattern. These cells remain on the outer surface of the chordal centra during mineralization as well as subsequent perichordal ossification of the vertebral bodies. Using twist1a1:dsRed and osx:mCherry transgenic lines we show that a subset of col10a1:nlGFP cells is derived from sclerotomal precursors and differentiates into future osteoblasts.
For the first time, this shows a segmental occurrence of putative osteoblast precursors in the vertebral centra prior to ossification of the notochordal sheath. This opens the possibility that sclerotome derived cells in teleosts are implicated in the establishment of the mineralized vertebral column in a similar manner as previously described for tetrapods.
Read online: Pubmed.
Learn more about Christoph Winkler’s research.